Coding

Part:BBa_K3747600:Design

Designed by: Sanne van Doorn   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nap_operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 235
    Illegal EcoRI site found at 844
    Illegal EcoRI site found at 2197
    Illegal SpeI site found at 2148
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 235
    Illegal EcoRI site found at 844
    Illegal EcoRI site found at 2197
    Illegal SpeI site found at 2148
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 235
    Illegal EcoRI site found at 844
    Illegal EcoRI site found at 2197
    Illegal BglII site found at 1838
    Illegal BglII site found at 3834
    Illegal XhoI site found at 416
    Illegal XhoI site found at 1519
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 235
    Illegal EcoRI site found at 844
    Illegal EcoRI site found at 2197
    Illegal SpeI site found at 2148
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 235
    Illegal EcoRI site found at 844
    Illegal EcoRI site found at 2197
    Illegal SpeI site found at 2148
    Illegal NgoMIV site found at 172
    Illegal NgoMIV site found at 1193
    Illegal NgoMIV site found at 1963
    Illegal NgoMIV site found at 2810
    Illegal NgoMIV site found at 3112
    Illegal NgoMIV site found at 3602
    Illegal AgeI site found at 655
    Illegal AgeI site found at 2659
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The structure of this Nap operon is napEDABC. More information for the subunits can be found here: napE:BBa_K3747100,napD:BBa_K3747101,napA:BBa_K3747102,napB:BBa_K3747103,napC:BBa_K3747104.

Source

Pseudomonas stutzeri JM300

References